Overview

Our laboratory investigates the genetic control mechanisms allowing the immune system to recognize and destroy foreign pathogens, but not normal constituents of the body. Defects in these mechanisms underlie many autoimmune diseases, including type 1 (juvenile onset, insulin dependent) diabetes. Using NOD (non-obese diabetic) inbred mice, we focus on the process through which genes that normally elicit immune responses to foreign intruders can sometimes trigger autoimmune responses against the body's own cells, such as the pancreatic cells that make insulin. We have shown that some genes play a role in type 1 diabetes even when they do not contain deleterious mutations. Instead they are normal genes that only contribute to type 1 diabetes pathology when collected together in a specific fashion. We are also investigating defects in the differentiation of a particular type of leukocyte in NOD mice that subsequently allows for the development of autoimmune responses. This work may help identify the mechanisms and compounds that normally prevent autoimmunity, and hence reveal strategies for pharmacological interventions in humans at risk for type 1 diabetes.

Scientific report

Genetic Basis of Immunological Tolerance Defects Underlying Type 1 Diabetes

Our primary research interest is understanding the genetic basis for immune tolerance to endogenous proteins. Defects in these mechanisms lead to many debilitating autoimmune diseases, of which type 1 diabetes (T1D) is one of the most serious. In both humans and NOD mice, T1D results when insulin-producing pancreatic ß-cells are destroyed by an autoreactive T-cell response. Thus, insights into the genetic mechanisms responsible for the normal maintenance of immunological tolerance can be gained by identifying the pathogenic basis of T1D in NOD mice.

T1D is controlled by a large number of susceptibility (Idd) genes. However, genes within particular major histocompatibility complexes (MHC) provide the primary component for T1D susceptibility in both humans and NOD mice. The MHC encodes two primary types of gene products, termed class I and class II. Class I molecules present peptides derived from intracellular proteins to CD8 T-cells, which usually exert cytotoxic functions. Virtually all cells express MHC class I molecules. In contrast, MHC class II expression is largely limited to a specialized subset of hematopoietically derived antigen presenting cells (APC) that include B-lymphocytes, macrophages, and dendritic cells (DC). MHC class II molecules expressed by APC display peptides derived from internalized extracellular proteins to CD4 T-cells, which produce cytokine molecules that amplify other components of the immune response, including cytotoxic CD8 T-cells.

T-cells specifically recognize a particular peptide/MHC antigenic complex through expression of clonally distributed T-cell receptor (TCR) molecules generated by somatically rearranged gene sequences. In addition to those derived from infectious pathogens, APC also display to T-cells MHC class I- and class II-bound peptides generated from endogenous "self" proteins. A normal consequence of expressing a TCR that engages "self antigenic" complexes on APC at a critically high threshold level is the induction of signals triggering the deletion or permanent inactivation of potentially autoreactive T-cells. Defects in this process of immunological tolerance induction manifested by both T-cells and APC underlie susceptibility to T1D.

In both humans and NOD mice, unusual MHC class II genes clearly contribute to T1D by inducing pathogenic CD4 T-cell responses. However, while representing common variants shared by many strains lacking autoimmune proclivity, the MHC class I molecules expressed by NOD mice also exert pathogenic functions essential to T1D development. These common class I variants aberrantly exert diabetogenic functions in NOD mice through interactions with some of this strain's multiple Idd genes located outside the MHC. The strongest such interactive effect is provided by a gene(s) within the Idd7 locus. The NOD Idd7 variant appears to prevent the TCR of autoreactive T-cells from being expressed at the level necessary to elicit a signaling response that is sufficiently strong to trigger the deletion of such pathogenic effectors upon their engagement of self antigen.

In humans, epidemiological studies also suggested that when co-expressed with particular combinations of other genes, some common MHC class I variants such as HLA-A2.1 could contribute to T1D development. To test this possibility, we generated NOD mice that transgenically express human HLA-A2.1, but no murine class I molecules. The human HLA-A2.1 molecules in these mice were found to mediate diabetogenic T-cell responses. Together with collaborators at The Albert Einstein College of Medicine, we identified three ß -cell autoantigenic peptides that are presented to diabetogenic T-cells by human HLA-A2.1 class I molecules in our transgenic mice. Subsequent work by another collaborator at Leiden University in The Netherlands revealed that at least one of these peptides is recognized in an HLA-A2.1-dependent fashion by CD8 T-cells isolated from a human T1D patient.

B-lymphocytes represent a critical subset of APC for activating the MHC class II-dependent T-cell responses contributing to T1D in NOD mice. The preferential ability of NOD B-lymphocytes to serve as diabetogenic APC results from the fact that they, unlike other types of APC, express plasma membrane-bound immunoglobulin (Ig) molecules which specifically capture and internalize ß -cell proteins. Mechanisms are normally in place to prevent the development of B-lymphocytes expressing autoreactive Ig molecules. We found that several of these mechanisms are defective in NOD mice. Further studies indicated that genes within the Idd5 and Idd9/11 loci contribute to the defective ability of NOD mice to inactivate B-lymphocytes expressing autoreactive Ig molecules.

The DC subset of APC in NOD mice is characterized by developmental defects preventing them from presenting self antigens in a sufficiently vigorous fashion to induce T-cell tolerogenic processes. Human T1D patients share similar DC developmental defects. Impaired DC differentiation in NOD mice is in turn linked to numerical deficiencies in the CD4-expressing subset of immunoregulatory natural killer (NK) T-cells that also characterize this strain. The loss of this NKT-cell subset appears to result from the fact that they are highly sensitive to apoptotic deletion when the cell surface enzyme ADP-ribosyltransferase 2 (ART2) is activated by its substrate NAD. Thus, blocking ART2 activity may have T1D protective effects in NOD mice by enhancing the survival of NKT-cells that in turn drive development of tolerogenic DC. However, due to its small size, and the fact that it is deeply imbedded in the plasma membrane, the catalytic site of ART2 cannot be targeted by conventional two-chain antibodies. However, llamas have the unusual feature of being able to generate single-chain antibodies that can target small, not readily accessible molecules. Early results from studies done in collaboration with investigators at the University of Hamburg in Germany indicate that treatment with a llama single-chain antibody can block ART2 activity in NOD mice resulting in enhanced numbers of NKT-cells and potentially tolerogenic DC. Further studies are ongoing to determine if blocking ART2 activity with this llama single-chain antibody has T1D protective effects in NOD mice.

Lab staff

Principal Investigator: David V. Serreze, Ph.D.
Associate Research Scientist:  Caroline McPhee, Ph.D.
Postdoctoral Fellow:  Maximiliano Fernando Presa, Ph.D.
Professional Assistant: Harold D. Chapman, B.S.
Research Assistant:
Deanna J. Lamont, B.S.
Laboratory Technician: Brenice Timms
Manager, Type 1 Diabetes Repos: Racheal Wallace
Research Administrative Assistant: Norma D. Buckley

Publication listings

2013

Presa M, Ortiz AZ, Garabatos N, Izquierdo C, Rivas EI, Teyton L, Mora C, Serreze D, Stratmann T.  2013. Cholera toxin subunit B-peptide fusion proteins reveal impaired oral tolerance induction in diabetes-prone but not in diabetes-resistant mice.  Eur J Immunol 43(11): 2969-2979. PMCID: PMC3856580

Mukherjee G, Geliebter A, Babad J, Santamaria P, Serreze DV, Freeman GJ, Tarbell KV, Sharpe A, Dilorenzo TP. 2013. DEC-205-mediated antigen Targeting to steady-state dendritic cells induces deletion of diabetogenic CD8+ T cells independently of PD-1 and PD-L1. Int Immunol 25(11): 651-660. PMCID: PMC3806169

2012

Lee JS, Scandiuzzi L, Ray A, Wei J, Hofmeyer KA, Abadi YM, Loke P, Lin J, Yuan J, Serreze DV, Allison JP, Zang X. 2012. B7x in the periphery abrogates pancreas-specific damage mediated by self-reactive CD8 T cells. J Immunol 189(8): 4165-4174. PMCID: PMC3466330

Stolp J, Chen YG, Cox SL, Henck V, Zhang W, Tsaih SW, Chapman H, Stearns T, Serreze DV, Silveira PA. 2012. Subcongenic analyses reveal complex interactions between distal chromosome 4 genes controlling diabetogenic B cells and CD4 T cells in nonobese diabetic mice. J Immunol 189(3): 1406-1417. PMCID: PMC3401322

Chen YG, Tsaih SW, Serreze DV. 2012. Genetic control of murine invariant natural killer T-cell development dynamically differs dependent on the examined tissue type. Genes Immun 13(2): 164-174. PMCID: PMC3291802

Wekerle H, Flugel A, Fugger L, Schett G, Serreze D. 2012. Autoimmunity's next top models. Nat Med 18(1): 66-70. Commentary

Unger WW, Pearson T, Abreu JR, Laban S, van der Slik AR, der Kracht SM, Kester MG, Serreze DV, Shultz LD, Griffioen M, Drijfhout JW, Greiner DL, Roep BO. 2012. Islet-specific CTL cloned from a Type 1 diabetes patient cause beta-cell destruction after engraftment into HLA-A2 transgenic NOD/SCID/IL2RG null mice. PLoS One 7(11): e49213. PMCID: PMC3498321

Bogdanik LP, Chapman HD, Miers KE, Serreze DV, Burgess RW. 2012. A MusD retrotransposon insertion in the mouse Slc6a5 gene causes alterations in neuromuscular junction maturation and behavioral phenotypes. PLoS One 7(1): e30217. PMCID: PMC3260239

2011

Chen YG, Scheuplein F, Driver JP, Hewes AA, Reifsnyder PC, Leiter EH, Serreze DV. 2011. Testing the role of P2X(7) receptors in the development of type 1 diabetes in nonobese diabetic mice. J Immunol 186:4278-4284. PMCID:  PMC3094905

Driver JP, Chen YG, Zhang W, Asrat S, Serreze DV. 2011. Unmasking genes in a type 1 diabetes-resistant mouse strain that enhances pathogenic CD8 T-cell responses. Diabetes 60:1354-1359. PMCID:  PMC3064110

Driver JP, Lamont DJ, Gysemans C, Mathieu C, Serreze DV. 2011. Calcium insufficiency accelerates type 1 diabetes in Vitamin D receptor-Deficient nonobese diabetic (NOD) mice. Endocrinology 152:4620-4629. PMCID: PMC3230053

Driver JP, Serreze DV, Chen YG. 2011. Mouse models for the study of autoimmune type 1 diabetes: a NOD to similarities and differences to human disease. Semin Immunopathol 33:67-87. Review

Niens M, Grier AE, Marron M, Kay TW, Greiner DL, Serreze DV. 2011. Prevention of "Humanized" diabetogenic CD8 T-cell responses in HLA-transgenic NOD mice by a multipeptide coupled-cell approach. Diabetes 60:1229-1236. PMCID:  PMC3064096

Rivas EI, Driver JP, Garabatos N, Presa M, Mora C, Rodriguez F, Serreze DV, Stratmann T. 2011. Targeting of a T cell agonist peptide to lysosomes by DNA vaccination induces tolerance in the nonobese diabetic mouse. J Immunol 186:4078-4087

Serreze DV, Chapman HD, Niens M, Dunn R, Kehry MR, Driver JP, Haller M, Wasserfall C, Atkinson MA. 2011. Loss of intra-islet CD20 expression may complicate efficacy of B-cell-directed Type 1 diabetes therapies. Diabetes 60(11): 2914-2921. PMCID:  PMC3198088

2010

Cox SL, Stolp J, Hallahan NL, Counotte J, Zhang W, Serreze DV, Basten A, Silveira PA. 2010. Enhanced responsiveness to T-cell help causes loss of B-lymphocyte tolerance to a beta-cell neo-self antigen in type 1 diabetes prone NOD mice. Eur J Immunol 40:3413-3425

Driver JP, Scheuplein F, Chen YG, Grier AE, Wilson SB, Serreze DV. 2010. Invariant natural killer T-cell control of type 1 diabetes: a dendritic cell genetic decision of a silver bullet or Russian roulette. Diabetes 59:423-432. PMCID: PMC2809954

Scheuplein F, Rissiek B, Driver JP, Chen YG, Koch-Nolte F, Serreze DV. 2010. A recombinant heavy chain antibody approach blocks ART2 mediated deletion of an iNKT cell population that upon activation inhibits autoimmune diabetes. J Autoimmun 34:145-154. PMCID: PMC2822066

Serreze DV, Niens M, Kulik J, Dilorenzo TP. 2010. Bridging mice to men: using HLA transgenic mice to enhance the future prediction and prevention of autoimmune type 1 diabetes in humans. Methods Mol Biol 602: 119-134 Book chapter

Strom A, Sonier B, Chapman HD, Mojibian M, Wang GS, Slatculescu CR, Serreze DV, Scott FW. 2010. Peripherin-reactive antibodies in mouse, rabbit, and human blood. J Proteome Res 9:1203-1208. PMC3023150

2009

Mangada J, Pearson T, Brehm MA, Wicker LS, Peterson LB, Shultz LD, Serreze DV, Rossini AA, Greiner DL. 2009. Idd loci synergize to prolong islet allograft survival induced by costimulation blockade in NOD mice. Diabetes 58(1):165-173. PMCID: PMC2606867

Wesolowski J, Alzogaray V, Reyelt J, Under M, Juarez K, Urrutia M, Cauerhff A, Danquah W, Rissiek B, Scheuplein F, Schwarz N, Adriouch S, Boyer O, Seman M, Licea A, Serreze DV, Goldbaum FA, Haag F, Koch-Nolte F. 2009. Single domain antibodies: promising experimental and therapeutic tools in infection and immunity. Med Microbiol Immunol 198:157-174. PMCID: PMC2714450.

2008

Chaparro RJ, Burton AR, Serreze DV, Vignali DA, Dilorenzo TP. 2008. Rapid identification of MHC class I-restricted antigens relevant to autoimmune diabetes using retrogenic T cells. J Immunol Methods 335:106-115. PMCID: PMC2430474

Chen YG, Scheuplein F, Osborne MA, Tsaih SW, Chapman HD, Serreze DV. 2008. Idd9/11 genetic locus regulates diabetogenic activity of CD4 T-cells in nonobese diabetic (NOD) mice. Diabetes 57:3273-3280. PMCID: PMC2584133

Driver JP, Foreman O, Mathieu C, van Etten E, Serreze DV. 2008. Comparative therapeutic effects of orally administered 1,25-dihydroxyvitamin D(3) and 1alpha-hydroxyvitamin D(3) on type-1 diabetes in non-obese diabetic mice fed a normal-calcaemic diet. Clin Exp Immunol 151:76-85. PMCID: PMC2276922

Mukhopadhaya A, Hanafusa T, Jarchum I, Chen YG, Iwai Y, Serreze DV, Steinman RM, Tarbell KV, DiLorenzo TP. 2008. Selective delivery of beta cell antigen to dendritic cells in vivo leads to deletion and tolerance of autoreactive CD8+ T cells in NOD mice. Proc Natl Acad Sci U S A 105:6374-6379. PMCID: PMC2359791

Serreze DV, Choisy-Rossi CM, Grier AE, Holl TM, Chapman HD, Gahagan JR, Osborne MA, Zhang W, King BL, Brown A, Roopenian D, Marron MP. 2008. Through Regulation of TCR Expression Levels, an Idd7 Region Gene(s) Interactively Contributes to the Impaired Thymic Deletion of Autoreactive Diabetogenic CD8+ T Cells in Nonobese Diabetic Mice. J Immunol 180:3250-3259.

2007

Chen YG, Driver JP, Silveira PA, Serreze DV. 2007. Subcongenic analysis of genetic basis for impaired development of invariant NKT cells in NOD mice. Immunogenetics 59:705-712.

Chen Y-G, Silveira PA, Osborne MA, Chapman HD, Serreze DV. 2007. Cellular expression requirements for inhibition of type 1 diabetes by a dominantly protective major histocompatibility complex haplotype. Diabetes 56:424-430.

Jarchum I, Baker JC, Yamada T, Takaki T, Marron MP, Serreze DV, DiLorenzo TP. 2007. In vivo cytotoxicity of insulin-specific CD8+ T-cells in HLA-A*0201 transgenic NOD mice. Diabetes 56:2551-2560.

Leiter EH, Reifsnyder P, Driver J, Kamdar S, Choisy-Rossi C, Serreze DV, Hara M, Chervonsky A. 2007. Unexpected functional consequences of xenogeneic transgene expression in beta-cells of NOD mice. Diabetes Obes Metab Suppl 2:14-22.

Serreze DV, Marron MP, Dilorenzo TP. 2007. "Humanized" HLA transgenic NOD mice to identify pancreatic beta cell autoantigens of potential clinical relevance to type 1 diabetes. Ann N Y Acad Sci 1103:103-111.

Serreze DV, Silveira PA. 2007. To be an autoreactive B (cell) or not to be: are lessons learned about this question in type 1 diabetes applicable to autoimmune thyroiditis. In: The Thyroid and Autoimmunity, Wiersinga WM, (ed). Thieme Medical Publishers, Incorporated, Stuttgart, Germany. pp. 104-109.

Silveira PA, Serreze DV, Grey ST. 2007. Invasion of the killer B's in type 1 diabetes. Front Biosci 12:2183-2193. Review

Unger WW, Pinkse GG, Mulder-van der Kracht S, van der Slik AR, Kester MG, Ossendorp F, Drijfhout JW, Serreze DV, Roep BO. 2007. Human clonal CD8 autoreactivity to an IGRP islet epitope shared between mice and men. Ann N Y Acad Sci 1103:192-195.

Yamanouchi J, Rainbow D, Serra P, Howlett S, Hunter K, Garner VE, Gonzalez-Munoz A, Clark J, Veijola R, Cubbon R, Chen SL, Rosa R, Cumiskey AM, Serreze DV, Gregory S, Rogers J, Lyons PA, Healy B, Smink LJ, Todd JA, Peterson LB, Wicker LS, Santamaria P. 2007. Interleukin-2 gene variation impairs regulatory T cell function and causes autoimmunity. Nat Genet 39:329-337. PMCID: PMC2886969

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