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Home  > Research and resources > Research areas and faculty > Kevin Mills, Ph.D. > Lab site

Protocols

PCR Protocols:
  • AID Genotyping
  • Artemis Genotyping
  • CD19 Cre Genotyping
  • CD21 Cre Genotyping
  • Conditional Xrcc2 Flox or Deleted genotyping
  • JH region Genotyping
  • Ligase4 Genotyping
  • p53r172 Genotyping
  • Rag1 Genotyping
  • Q-PCR, SYBR Green Method
  • RT-PCR, using Qiagen Onestep Kit
  • Trp53 Genotyping
  • Xrcc2 Genotyping
Flow Cytometry Protocols:
  • Annexin V
  • Propidium Iodide Cell Cycle Analysis
  • Micronuclei Analysis
Cell Culture Protocols:
  • Fetal Liver Culture
  • Primary MEF Isolation
  • Murine Splenocyte Class Switch Recombination
  • Cryopreservation
  • Mesenchymal Stem Cell Isolation
  • Genomic DNA preparation
  • CLL Sample Receipt and Processing
  • MTS Cell Proliferation Assay
Cytogenetic Protocols:
  • Metaphase Preparation Optimized B&T cells
  • Metaphase Preparation optimized for pro-B Cells
  • Metaphase Drop
  • General Metaphase Preparation
  • Spectral Karyotyping-- Mouse
  • BioPrime Biotin Labeling (FISH)
  • FISH Probe Preparation
  • FISH
  • Direct Fluorescent Labeling
Protein Expression Protocols:
  • Bradford Method
  • Coomasie Protein Stain
  • Whole Cell Extract Preparation
  • Western Blot
  • Whole B-Cell ELISA
  • Serum ELISA
    Molecular Biology Protocols:
  • Junction Cloning
  • Lentiviral Transfection
  • Qiagen Midi Prep, optimized for BAC DNA
  • RNA Isolation, using RNeasy kit
  • Southern Blot
Immunofluorescence Protocols:
  • Immunofluorescence of Frozen Tissue
  • Immunofluorescence of MEFs
  • Immunofluorescence of Parrafin Embedded Tissue Sections
  • Immunofluorescence of Primary Splenocytes
  • Poly-L-Lysine Coating of Coverslips
  • Back to Faculty information

In this section

  • Mills Lab
    • Lab Personnel
    • Research Description
    • Protocols
    • Publications
    • SKY
    • Recruiting
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