Protocols
PCR Protocols:- AID Genotyping
- Artemis Genotyping
- CD19 Cre Genotyping
- CD21 Cre Genotyping
- Conditional Xrcc2 Flox or Deleted genotyping
- JH region Genotyping
- Ligase4 Genotyping
- p53r172 Genotyping
- Rag1 Genotyping
- Q-PCR, SYBR Green Method
- RT-PCR, using Qiagen Onestep Kit
- Trp53 Genotyping
- Xrcc2 Genotyping
- Fetal Liver Culture
- Primary MEF Isolation
- Murine Splenocyte Class Switch Recombination
- Cryopreservation
- Mesenchymal Stem Cell Isolation
- Genomic DNA preparation
- CLL Sample Receipt and Processing
- MTS Cell Proliferation Assay
- Metaphase Preparation Optimized B&T cells
- Metaphase Preparation optimized for pro-B Cells
- Metaphase Drop
- General Metaphase Preparation
- Spectral Karyotyping-- Mouse
- BioPrime Biotin Labeling (FISH)
- FISH Probe Preparation
- FISH
- Direct Fluorescent Labeling
- Bradford Method
- Coomasie Protein Stain
- Whole Cell Extract Preparation
- Western Blot
- Whole B-Cell ELISA
- Serum ELISA
- Molecular Biology Protocols:
