Spectral Karyotyping (SKY)
We use the mouse spectral karyotyping platform from Applied Spectral Imaging. This system uses combinatorial chromosome paints to uniquely label each mouse chromosome. Spectral detection is carried out using ASI’s SKY imaging system to combine spectrometry with CCD-based monochrome. To perform chromosome classification each unique spectral signature is then assigned a different color, and these colors are mapped to those pixels in the image containing the corresponding spectrum. See the Applied Spectral Imaging Technology page for a description of their SpectraCube® technology.
Spectral karyotype (SKY) analysis of bone marrow cell with two translocations. Total bone marrow isolated from a normal mouse was exposed to 1Gy ionizing radiation, allowed to recover for 24 hours, and anlayzed by SKY, revealing two transloctions: a dicentric translocation involving chromosomes 2 and 14; and a simple, non-reciprocal translocation involving chromosomes 2 and 19. (Imaging and analysis by Anthony Britt)
Spectral karyotype (SKY) analysis of bone marrow from a Pohn mouse more than 20 months of age. A) Spectral image of a single metaphase spread. B) Classified image derived from spectral data shown in A. C) Karyotype table showing a total of 41 chromosomes, but aneusomy for chromosomes 4, 5, 7, 10, 15, 17, 18, 19, and the sex chromosomes. Arrows mark duplicated chromosome 2. (Imaging and analysis by Sarah Wright).
