Knockout Mouse Project (KOMP)

Since 2006, scientists around the world have been working toward the goal of generating a targeted knockout mutation for every gene in the mouse genome.  These knockout mice will create a scientific resource of critical tools for understanding gene function and the genetic causes of human diseases.  Read more about KOMP

With ES cells for over 14,000 gene knockouts in embryonic stem (ES) cells complete, the international scientific community has embarked on the second phase of this project: generating and phenotyping mice for each targeted gene.  In the United States, the National Institutes of Health has funded three “KOMP2” centers, including one at The Jackson Laboratory, to work together on the immense task of producing and phenotyping these mice. Combined with the international effort coordinated by the International Mouse Phenotyping Consortium (IMPC), a total of 5,000 knockout mice will be produced and phenotyped by 2016. We are pleased to contribute to this international effort to establish a global resource of knockout mice and related database of gene function.

Production of mice from ES cells

As part of the KOMP2 program, we will be generating cohorts of 833 strains of knockout mice from ES cells over the next 5 years. We will determine viability and fecundity for each strain, as well as the expression pattern for the targeted gene, and will cryopreserve germplasm for future researchers to use. In the process we will focus on developing new technologies that reduce the costs and increase the efficiency of mouse production. Learn more about KOMP mice production

Phenotyping mice to determine gene function

Once mouse colonies are established, all mice will be phenotyped in a rigorous screening program that will examine multiple organ systems to evaluate gene function for each knockout mutation.  Phenotypic data will be assessed both quantitatively and qualitatively, with bi-weekly “Grand Rounds” in which specialists from diverse fields appraise the collected data. Learn more about the phenotyping of KOMP mice